Antibodies directed towards CD22 have been utilized in radioimmunotherapy (RIT) scientific trials to deal with sufferers with diffuse giant B-cell lymphoma (DLBCL) with promising outcomes. However, related preclinical fashions are wanted to facilitate the analysis and optimization of new protocols. Spontaneous DLBCL in canine is a tumor mannequin that will assist speed up the improvement of new methodologies and therapeutic methods for RIT concentrating on CD22.
Seven murine monoclonalantibodies particular for canine CD22 had been produced by the hybridoma technique and characterised. The antibodies‘ affinity and epitopic maps, their internalization functionality and usefulness for prognosis in immunohistochemistry had been decided.
Biodistribution and PET imaging on a mouse xenogeneic mannequin of canine DLBCL was used to decide on the most promising antibody for our functions. PET-CT outcomes confirmed biodistribution examine observations and allowed tumor localization. The chosen antibody, 10C6, was efficiently used on a canine with spontaneous DLBCL for SPECT-CT imaging in the context of illness staging, validating its efficacy for prognosis and the feasibility of future RIT assays.
SPECT-CT Imaging of Dog Spontaneous Diffuse Large B-Cell Lymphoma Targeting CD22 for the Implementation of a Relevant Preclinical Model for Human.
Description: A polyclonal antibody against NIM1K. Recognizes NIM1K from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
Description: A polyclonal antibody against NIM1K. Recognizes NIM1K from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000
Description: A polyclonal antibody for detection of NIM1 from Human, Mouse. This NIM1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NIM1 at AA rangle: 320-400
Description: A polyclonal antibody for detection of NIM1 from Human, Mouse. This NIM1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NIM1 at AA rangle: 320-400
Description: A polyclonal antibody for detection of NIM1 from Human, Mouse. This NIM1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NIM1 at AA rangle: 320-400
Description: A polyclonal antibody against NIM1K. Recognizes NIM1K from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NIM1K. Recognizes NIM1K from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against NIM1K. Recognizes NIM1K from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Mouse Nim1 (C-term). This antibody is tested and proven to work in the following applications:
Description: Pre-made tet-inducible lentiviral particles expressing a human gene with a Blasticidin-RFP fusion marker (Dual selection). The expressed human gene, NIM1, is fully sequence verified and matched to NCBI accession ID: NM_153361
Description: Nimesulide is a relatively COX-2 selective, non-steroidal anti-inflammatory drug (NSAID) with analgesic and antipyretic properties.Nimesulide is a selective COX-2 inhibitor used in a variety of inflammatory, pain and fever states. After healthy volunteers
Description: Nimesulide is a relatively COX-2 selective, non-steroidal anti-inflammatory drug (NSAID) with analgesic and antipyretic properties.Nimesulide is a selective COX-2 inhibitor used in a variety of inflammatory, pain and fever states. After healthy volunteers
Description: Nimesulide is a relatively COX-2 selective, non-steroidal anti-inflammatory drug (NSAID) with analgesic and antipyretic properties.Nimesulide is a selective COX-2 inhibitor used in a variety of inflammatory, pain and fever states. After healthy volunteers
This first try at phenotypic imaging on canine paves the solution to implementing quantitative imaging methodologies that might be transposable to people in a theranostic strategy. Taking under consideration the suggestions of present human radioimmunotherapy scientific trials concentrating on CD22, animal trials are deliberate to analyze protocol enhancements which can be troublesome to think about in people as a result of moral considerations.
