Rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal antibodies against DKK2 for cancer therapy.

Dickkopf-related protein 2 (DKK2)is a member of the Dickkopf household in Wnt signaling pathway. Recently, we discovered that antibodies against DKK2 might activate pure killer (NK) and CD8+ T cells in tumors and inhibit tumor development. In this paper, we report the rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal anti-DKK2 antibodies.

To break the immune tolerance, we designed and chemically synthesized six peptides akin to completely different areas of DKK2 as immunogens and discovered 5 of them might generate mouse polyclonal antibodies that may bind to the lively recombinant human DKK2 protein.

Nicotinamide

HY-B0150 10mM/1mL
EUR 135.6

Nicotinamide

N1651-1000 1g
EUR 38
Description: PARP-1 inhibitor

Nicotinamide

N1651-5.1 10 mM (in 1mL DMSO)
EUR 64
Description: PARP-1 inhibitor

Nicotinamide

N1651-50 50 mg
EUR 157.2
Description: IC50: N/ANicotinamide is an inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. Poly(ADP-ribose) polymerase-1 (PARP-1) is found as a DNA repair enzyme.

Nicotinamide

N1651-5000 5g
EUR 50
Description: PARP-1 inhibitor

Nicotinamide

GP9253-100G 100 g
EUR 54

Nicotinamide

GP9253-1KG 1 kg
EUR 112.8

Nicotinamide

GP9253-250G 250 g
EUR 64.8

Nicotinamide

GP9253-25G 25 g
EUR 48

Nicotinamide

GP9253-500G 500 g
EUR 84

Nicotinamide

N407750 100g
EUR 75
Description: 98-92-0

Nicotinamide

NB0659 250g
EUR 84.01

Nicotinamide

T0934-10mg 10mg Ask for price
Description: Nicotinamide

Nicotinamide

T0934-1g 1g Ask for price
Description: Nicotinamide

Nicotinamide

T0934-1mg 1mg Ask for price
Description: Nicotinamide

Nicotinamide

T0934-50mg 50mg Ask for price
Description: Nicotinamide

Nicotinamide

T0934-5mg 5mg Ask for price
Description: Nicotinamide

Nicotinamide

abx184742-96tests 96 tests
EUR 81.25

Nicotinamide

TB0209 10X20mg
EUR 328.8

Nicotinamide

MBS577577-1g 1g
EUR 145

Nicotinamide

MBS577577-500mg 500mg
EUR 145

Nicotinamide

MBS577577-5x1g 5x1g
EUR 505

Nicotinamide

MBS696423-100mg 100mg
EUR 155

Nicotinamide

MBS696423-50mg 50mg
EUR 130

Nicotinamide

MBS696423-5x100mg 5x100mg
EUR 650

Nicotinamide

MBS3606896-100mg 100mg
EUR 225

Nicotinamide

MBS3606896-10mg 10mg
EUR 185

Nicotinamide

MBS3606896-200mg 200mg
EUR 245

Nicotinamide

MBS3606896-25mg 25mg
EUR 200

Nicotinamide

MBS3606896-50mg 50mg
EUR 215

Nicotinamide

MBS565445-05mL 0.5mL
EUR 150

Nicotinamide

MBS565445-5x05mL 5x0.5mL
EUR 525

Nicotinamide-13C6

N407754 25mg
EUR 4500

Nicotinamide-13C6

HY-B0150S2 1 mg
EUR 2002.2
Description: Nicotinamide-13C6 is the 13C-labeled Nicotinamide. Nicotinamide is a form of vitamin B3 that plays essential roles in cell physiology through facilitating NAD+ redox homeostasis and providing NAD+ as a substrate to a class of enzymes that catalyze non-redox reactions. Nicotinamide is an inhibitor of SIRT1.

Nicotinamide-13C3,15N

V125450 100mg
EUR 12800
Description: 2117346-85-5

Nicotinamide, 99%

GP9346 25g
EUR 57.9

Nicotinamide 99%

N05200 100G
EUR 215.5

Nicotinamide, 99%

GP9346-1 1
EUR 63.3

Nicotinamide, 99%

GP9346-100 100
EUR 16.4

Nicotinamide, 99%

GP9346-25 25
EUR 10.3

Nicotinamide, 99%

GP9346-250 250
EUR 23.8

Nicotinamide, 99%

GP9346-500 500
EUR 39.4

Nicotinamide [BSA]

DAG3371 1mg
EUR 1014

Nicotinamide-d4

464819 1.0mg
EUR 250

Nicotinamide-d4

N407752 2.5mg
EUR 1510
Description: 347841-88-7

Nicotinamide-d4

HY-B0150S 10 mg
EUR 346.33
Description: Nicotinamide-d4 is the deuterium labeled Nicotinamide. Nicotinamide is a form of vitamin B3 that plays essential roles in cell physiology through facilitating NAD+ redox homeostasis and providing NAD+ as a substrate to a class of enzymes that catalyze non-redox reactions. Nicotinamide is an inhibitor of SIRT1.

Nicotinamide-15N,13C3

HY-B0150S1 1mg Ask for price
Description: Nicotinamide-15N,13C3 is the 13C-labeled and 15N-labeled Nicotinamide. Nicotinamide is a form of vitamin B3 that plays essential roles in cell physiology through facilitating NAD+ redox homeostasis and providing NAD+ as a substrate to a class of enzymes that catalyze non-redox reactions. Nicotinamide is an inhibitor of SIRT1.

Nicotinamide riboside

T13795-10mg 10mg Ask for price
Description: Nicotinamide riboside

Nicotinamide riboside

T13795-1g 1g Ask for price
Description: Nicotinamide riboside

Nicotinamide riboside

T13795-1mg 1mg Ask for price
Description: Nicotinamide riboside

Nicotinamide riboside

T13795-50mg 50mg Ask for price
Description: Nicotinamide riboside

Nicotinamide riboside

T13795-5mg 5mg Ask for price
Description: Nicotinamide riboside

Nicotinamide riboside

MBS5754351-5mg 5mg
EUR 125

Nicotinamide riboside

MBS5754351-5x5mg 5x5mg
EUR 405

Nicotinamide (Standard)

HY-B0150R Get quote Ask for price
Description: Nicotinamide (Standard) is the analytical standard of Nicotinamide. This product is intended for research and analytical applications. Nicotinamide is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide also inhibits SIRT1. Nicotinamide increases cellular NAD+, ATP, ROS levels. Nicotinamide inhibits tumor growth and improves survival. Nicotinamide also has anti-HBV activity[1][2][3][4].

Nicotinamide riboside

HY-123033 Get quote Ask for price
Description: Nicotinamide riboside, an orally active NAD+ precursor, increases NAD+ levels and activates SIRT1 and SIRT3. Nicotinamide riboside is a source of vitamin B3 (niacin) and enhances oxidative metabolism, protection against high fat diet-induced metabolic abnormalities[1]. Nicotinamide riboside reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease[2].

Nicotinamide ascorbate

T33662-10mg 10mg Ask for price
Description: Nicotinamide ascorbate

Nicotinamide ascorbate

T33662-1g 1g Ask for price
Description: Nicotinamide ascorbate

Nicotinamide ascorbate

T33662-1mg 1mg Ask for price
Description: Nicotinamide ascorbate

Nicotinamide ascorbate

T33662-50mg 50mg Ask for price
Description: Nicotinamide ascorbate

Nicotinamide ascorbate

T33662-5mg 5mg Ask for price
Description: Nicotinamide ascorbate

Nicotinamide ascorbate

MBS5783204-1g 1(g
EUR 465

Nicotinamide ascorbate

MBS5783204-5mg 5mg
EUR 915

Nicotinamide ascorbate

MBS5783204-5x1g 5x1g
EUR 1945

Nicotinamide ascorbate

MBS5783204-5x5mg 5x5mg
EUR 3970

Nicotinamide (Niacinamide)

PCT0209-100G 1 unit
EUR 6.93
Description: Nicotinamide (Niacinamide)

Nicotinamide N-oxide

C6736-100 100mg
EUR 40

Nicotinamide N-oxide

C6736-200 200mg
EUR 68

Nicotinamide N-oxide

HY-101407 10mM/1mL
EUR 151.2

Nicotinamide-N-15N

N407751 50mg
EUR 800
Description: 113950-01-9

Nicotinamide N-Oxide

N407760 100mg
EUR 1006
Description: 1986-81-8

Nicotinamide N-oxide

T0617-10mg 10mg Ask for price
Description: Nicotinamide N-oxide

Nicotinamide N-oxide

T0617-1g 1g Ask for price
Description: Nicotinamide N-oxide

Nicotinamide N-oxide

T0617-1mg 1mg Ask for price
Description: Nicotinamide N-oxide

Nicotinamide N-oxide

T0617-50mg 50mg Ask for price
Description: Nicotinamide N-oxide

Nicotinamide N-oxide

T0617-5mg 5mg Ask for price
Description: Nicotinamide N-oxide

Nicotinamide N-Oxide

MBS6095116-100mg 100(mg
EUR 485

Nicotinamide N-Oxide

MBS6095116-5x100mg 5x100mg
EUR 2025

Nicotinamide N-oxide

MBS3844976-100mg 100mg
EUR 140

Nicotinamide N-oxide

MBS3844976-500mg 500mg
EUR 250

Nicotinamide N-oxide

MBS3844976-5x500mg 5x500mg
EUR 1110

Nicotinamide-d4 (Major)

N407753 100mg
EUR 249
Description: 347841-88-7

Nicotinamide-d4 (Major)

MBS6058770-10mg 10(mg
EUR 590

Nicotinamide-d4 (Major)

MBS6058770-5x10mg 5x10mg
EUR 2500

Nicotinamide (Hydrochloride)

HY-B0150A Get quote Ask for price
Description: Nicotinamide Hydrochloride is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide Hydrochloride also inhibits SIRT1. Nicotinamide Hydrochloride increases cellular NAD+, ATP, ROS levels. Nicotinamide Hydrochloride inhibits tumor growth and improves survival. Nicotinamide Hydrochloride also has anti-HBV activity[1][2][3][4].

Nicotinamide-N-oxide

MBS3603105-100mg 100mg
EUR 200

Nicotinamide-N-oxide

MBS3603105-200mg 200mg
EUR 225

Nicotinamide-N-oxide

MBS3603105-5x200mg 5x200mg
EUR 700

Nicotinamide-N-oxide

MBS576693-100mg 100mg
EUR 145

Nicotinamide-N-oxide

MBS576693-200mg 200mg
EUR 165

Nicotinamide-N-oxide

MBS576693-5x200mg 5x200mg
EUR 585

?-Nicotinamide mononucleotide

2733-100 each
EUR 418.8

?-Nicotinamide mononucleotide

2733-25 each
EUR 196.8

?-Nicotinamide mononucleotide

2733-250 each
EUR 757.2

Nicotinamide Mononucleotide

330188 5.0g
EUR 150

Nicotinamide extrapure, 99.5%

72860 100 Gms
EUR 2.93
Description: Part B

Nicotinamide riboside malate

T40060-10mg 10mg Ask for price
Description: Nicotinamide riboside malate

Nicotinamide riboside malate

T40060-1g 1g Ask for price
Description: Nicotinamide riboside malate

Nicotinamide riboside malate

T40060-1mg 1mg Ask for price
Description: Nicotinamide riboside malate

Nicotinamide riboside malate

T40060-50mg 50mg Ask for price
Description: Nicotinamide riboside malate

Nicotinamide riboside malate

T40060-5mg 5mg Ask for price
Description: Nicotinamide riboside malate

Nicotinamide riboside malate

MBS5800375-5mg 5(mg
EUR 915

Nicotinamide riboside malate

MBS5800375-5x5mg 5x5(mg
EUR 3970

Nicotinamide riboside (malate)

HY-123033C Get quote Ask for price
Description: Nicotinamide riboside malate, an orally active NAD+ precursor, increases NAD+ levels and activates SIRT1 and SIRT3. Nicotinamide riboside malate is a source of vitamin B3 (niacin) and enhances oxidative metabolism, protection against high fat diet-induced metabolic abnormalities[1]. Nicotinamide riboside malate reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease[2].

Nicotinamide (Vitamin B3) USP

N018-100G 100 g
EUR 17.2
Description: C6H6N2O

Nicotinamide (Vitamin B3) USP

N018-500G 500 g
EUR 80.59
Description: C6H6N2O

Nicotinamide, N-(2-pyridyl)-

T33664-10mg 10mg Ask for price
Description: Nicotinamide, N-(2-pyridyl)-

Nicotinamide, N-(2-pyridyl)-

T33664-1g 1g Ask for price
Description: Nicotinamide, N-(2-pyridyl)-

Nicotinamide, N-(2-pyridyl)-

T33664-1mg 1mg Ask for price
Description: Nicotinamide, N-(2-pyridyl)-

Nicotinamide, N-(2-pyridyl)-

T33664-50mg 50mg Ask for price
Description: Nicotinamide, N-(2-pyridyl)-

Nicotinamide, N-(2-pyridyl)-

T33664-5mg 5mg Ask for price
Description: Nicotinamide, N-(2-pyridyl)-

Nicotinamide, N-(2-pyridyl)-

MBS5783206-INQUIRE INQUIRE Ask for price

Nicotinamide-d4 N-Oxide

MBS6024891-5mg 5(mg
EUR 610

Nicotinamide-d4 N-Oxide

MBS6024891-5x5mg 5x5(mg
EUR 2600

N-(Hydroxymethyl)nicotinamide

H948100 10g
EUR 75

Reduced Nicotinamide Riboside

R207800 100mg
EUR 3000
Description: 19132-12-8

N-(Hydroxymethyl)nicotinamide

T7869-10mg 10mg Ask for price
Description: N-(Hydroxymethyl)nicotinamide

N-(Hydroxymethyl)nicotinamide

T7869-1g 1g Ask for price
Description: N-(Hydroxymethyl)nicotinamide

N-(Hydroxymethyl)nicotinamide

T7869-1mg 1mg Ask for price
Description: N-(Hydroxymethyl)nicotinamide

N-(Hydroxymethyl)nicotinamide

T7869-50mg 50mg Ask for price
Description: N-(Hydroxymethyl)nicotinamide

N-(Hydroxymethyl)nicotinamide

T7869-5mg 5mg Ask for price
Description: N-(Hydroxymethyl)nicotinamide

N-(Hydroxymethyl)nicotinamide

HY-116993 5 g
EUR 54.11
Description: N-(Hydroxymethyl)nicotinamide is an antimicrobic agent[1].

Nicotinamide Riboside Chloride

329479 1.0g
EUR 150

Nicotinamide Riboside Triflate

564087 5.0mg
EUR 550

Nicotinamide riboside tartrate

T40059-10mg 10mg Ask for price
Description: Nicotinamide riboside tartrate

Nicotinamide riboside tartrate

T40059-1g 1g Ask for price
Description: Nicotinamide riboside tartrate

Nicotinamide riboside tartrate

T40059-1mg 1mg Ask for price
Description: Nicotinamide riboside tartrate

Nicotinamide riboside tartrate

T40059-50mg 50mg Ask for price
Description: Nicotinamide riboside tartrate

Nicotinamide riboside tartrate

T40059-5mg 5mg Ask for price
Description: Nicotinamide riboside tartrate

Nicotinamide riboside (chloride)

HY-123033A 100mg
EUR 129.6

Nicotinamide Riboside Chloride

N412460 1g
EUR 88
Description: 23111-00-4

Nicotinamide Riboside Triflate

T33663-10mg 10mg Ask for price
Description: Nicotinamide Riboside Triflate

Nicotinamide Riboside Triflate

T33663-1g 1g Ask for price
Description: Nicotinamide Riboside Triflate

Nicotinamide Riboside Triflate

T33663-1mg 1mg Ask for price
Description: Nicotinamide Riboside Triflate

Nicotinamide Riboside Triflate

T33663-50mg 50mg Ask for price
Description: Nicotinamide Riboside Triflate

Nicotinamide Riboside Triflate

T33663-5mg 5mg Ask for price
Description: Nicotinamide Riboside Triflate

Nicotinamide riboside chloride

T6220-10mg 10mg Ask for price
Description: Nicotinamide riboside chloride

Nicotinamide riboside chloride

T6220-1g 1g Ask for price
Description: Nicotinamide riboside chloride

Nicotinamide riboside chloride

T6220-1mg 1mg Ask for price
Description: Nicotinamide riboside chloride

Nicotinamide riboside chloride

T6220-50mg 50mg Ask for price
Description: Nicotinamide riboside chloride

Nicotinamide riboside chloride

T6220-5mg 5mg Ask for price
Description: Nicotinamide riboside chloride

Nicotinamide riboside tartrate

MBS5800374-5mg 5(mg
EUR 915

Nicotinamide riboside tartrate

MBS5800374-5x5mg 5x5(mg
EUR 3970

Nicotinamide Riboside Triflate

MBS5783205-5mg 5(mg
EUR 915

Neutralizing mousemonoclonalantibodies (5F8 and 1A10) against human DKK2 have been efficiently developed by immunizing the mice with two completely different peptides (34KLNSIKSSL42 and 240KVWKDATYS248) conjugated to Keyhole limpet hemocyanin (KLH). The monoclonalantibodies not solely abolish DKK2’s suppression of Wnt signaling in vitro but additionally inhibits tumor development in vivo. Currently, these two mAbs are present process humanization as immunotherapy candidates and could provide a brand new drug for remedy of human cancers.

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