Rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal antibodies against DKK2 for cancer therapy.

Dickkopf-related protein 2 (DKK2)is a member of the Dickkopf household in Wnt signaling pathway. Recently, we discovered that antibodies against DKK2 might activate pure killer (NK) and CD8+ T cells in tumors and inhibit tumor development. In this paper, we report the rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal anti-DKK2 antibodies.

To break the immune tolerance, we designed and chemically synthesized six peptides akin to completely different areas of DKK2 as immunogens and discovered 5 of them might generate mouse polyclonal antibodies that may bind to the lively recombinant human DKK2 protein.

Nicotinamide

GP9253-1KG 1 kg
EUR 94

Nicotinamide

GP9253-250G 250 g
EUR 54

Nicotinamide

GP9253-25G 25 g
EUR 40

Nicotinamide

GP9253-500G 500 g
EUR 70

Nicotinamide

N1651-5.1 10 mM (in 1mL DMSO)
EUR 142
Description: IC50: N/ANicotinamide is an inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. Poly(ADP-ribose) polymerase-1 (PARP-1) is found as a DNA repair enzyme.

Nicotinamide

N1651-50 50 mg
EUR 131
Description: IC50: N/ANicotinamide is an inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. Poly(ADP-ribose) polymerase-1 (PARP-1) is found as a DNA repair enzyme.

Nicotinamide

TB0209 10X20mg
EUR 274

Nicotinamide [BSA]

DAG3371 1mg
EUR 845

?-Nicotinamide mononucleotide

2733-100
EUR 349

?-Nicotinamide mononucleotide

2733-25
EUR 164

?-Nicotinamide mononucleotide

2733-250
EUR 631

Nicotinamide N-oxide

HY-101407 10mM/1mL
EUR 126

Nicotinamide riboside (chloride)

HY-123033A 100mg
EUR 108

β-Nicotinamide mononucleotide

HY-F0004 100mg
EUR 312

Beta-Nicotinamide Mononucleotide

abx188614-100g 100 g
EUR 1469

beta-Nicotinamide mononucleotide

GE9996-100MG 100 mg
EUR 138

beta-Nicotinamide mononucleotide

B7878-100 100 mg
EUR 108

beta-Nicotinamide mononucleotide

B7878-500 500 mg
EUR 166

Beta-Nicotinamide Mononucleotide

TBW01655 20mg Ask for price

Nicotinamide (Vitamin B3) USP

N018-100G 100 g
EUR 50

Nicotinamide (Vitamin B3) USP

N018-500G 500 g
EUR 123

Nicotinamide N-Methyltransferase Antibody

20-abx114105
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Nicotinamide Nucleotide Transhydrogenase Antibody

20-abx114107
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Nicotinamide N-Methyltransferase (Recombinant)

20-abx073298
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

Nicotinamide Adenine Dinucleotide (BSA)

20-abx165783
  • EUR 634.00
  • EUR 272.00
  • EUR 1901.00
  • EUR 746.00
  • EUR 453.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide Adenine Dinucleotide (OVA)

20-abx165784
  • EUR 634.00
  • EUR 272.00
  • EUR 1901.00
  • EUR 746.00
  • EUR 453.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

beta-Nicotinamide adenine dinucleotide

B7877-100 100 mg
EUR 108

beta-Nicotinamide adenine dinucleotide

B7877-500 500 mg
EUR 166

beta-Nicotinamide adenine dinucleotide

GV0860-10G 10 g
EUR 174

beta-Nicotinamide adenine dinucleotide

GV0860-1G 1 g
EUR 58

beta-Nicotinamide adenine dinucleotide

GV0860-25G 25 g
EUR 329

beta-Nicotinamide adenine dinucleotide

GV0860-5G 5 g
EUR 112

Nicotinamide Adenine Dinucleotide (NAD) Antibody

20-abx130456
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx122668-100ug 100 ug
EUR 391

Nicotinamide Nucleotide Adenylyltransferase 1 Antibody

20-abx137248
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Nicotinamide-N-Methyltransferase (NNMT) Antibody

20-abx103358
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide-N-Methyltransferase (NNMT) Antibody

20-abx103359
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide-N-Methyltransferase (NNMT) Antibody

20-abx103360
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide Nucleotide Adenylyltransferase 1 (Recombinant)

20-abx073291
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Nicotinamide Nucleotide Adenylyltransferase 2 (Recombinant)

20-abx073965
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody

20-abx007786
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody

20-abx003424
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Nicotinamide N-Methyltransferase (NNMT) Antibody

20-abx005116
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx025652-400ul 400 ul
EUR 523

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx025652-80l 80 µl
EUR 286

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx027598-400ul 400 ul
EUR 523

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx027598-80l 80 µl
EUR 286

Recombinant Human Nicotinamide N-Methyltransferase

7-03229 5µg Ask for price

Recombinant Human Nicotinamide N-Methyltransferase

7-03230 25µg Ask for price

Recombinant Human Nicotinamide N-Methyltransferase

7-03231 1mg Ask for price

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx431457-200ul 200 ul
EUR 384

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx433044-200ul 200 ul
EUR 384

Nicotinamide N-Methyltransferase (NNMT) Antibody

20-abx301014
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nicotinamide N-Methyltransferase (NNMT) Antibody

20-abx321820
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

N-Benzyl-5-bromo-nicotinamide

20-abx186522
  • EUR 217.00
  • EUR 648.00
  • EUR 328.00
  • 1 g
  • 25 g
  • 5 g

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody

20-abx217198
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody

abx146315-100ug 100 ug
EUR 391

Nicotinamide N-Methyltransferase (NNMT) Antibody

abx235773-100ug 100 ug
EUR 509

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody

abx235775-100ug 100 ug
EUR 481

Nicotinamide N-Methyltransferase (NNMT) Antibody

20-abx141366
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

Nicotinamide-N-Methyltransferase (NNMT) Antibody

20-abx173825
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Human Nicotinamide N-methyltransferase (NNMT)

1-CSB-EP015906HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Nicotinamide N-methyltransferase(NNMT) expressed in E.coli

Recombinant Nicotinamide-N-Methyltransferase (NNMT)

4-RPH530Hu01
  • EUR 490.66
  • EUR 234.00
  • EUR 1564.96
  • EUR 588.32
  • EUR 1076.64
  • EUR 391.00
  • EUR 3762.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Nicotinamide-N-Methyltransferase expressed in: E.coli

Recombinant Nicotinamide-N-Methyltransferase (NNMT)

4-RPH530Mu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Nicotinamide-N-Methyltransferase expressed in: E.coli

Recombinant Nicotinamide-N-Methyltransferase (NNMT)

4-RPH530Mu02
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Nicotinamide-N-Methyltransferase expressed in: E.coli

?-Nicotinamide adenine dinucleotide sodium salt

9457-1G
EUR 109

?-Nicotinamide adenine dinucleotide sodium salt

9457-25G
EUR 762

?-Nicotinamide adenine dinucleotide sodium salt

9457-5G
EUR 251

Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit

20-abx585168
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

NNMTMouse Nicotinamide N-Methyltransferase MouseRecombinant Protein

PROTO55239 Regular: 10ug
EUR 317
Description: NNMT Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids (1-264a.a) and having a molecular mass of 32.1kDa.;NNMT is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Pig NAMPT/ Nicotinamide phosphoribosyltransferase ELISA Kit

E0138Pi 1 Kit
EUR 717

Mouse Nampt/ Nicotinamide phosphoribosyltransferase ELISA Kit

E1008Mo 1 Kit
EUR 571

Rat Nampt/ Nicotinamide phosphoribosyltransferase ELISA Kit

E0659Ra 1 Kit
EUR 571

Human NAMPT/ Nicotinamide phosphoribosyltransferase ELISA Kit

E2812Hu 1 Kit
EUR 563

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody

abx122119-100ug 100 ug
EUR 391

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody

abx122669-100ug 100 ug
EUR 391

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody

20-abx114106
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Human Nicotinamide-N-Methyltransferase (NNMT) Protein

20-abx068292
  • EUR 690.00
  • EUR 286.00
  • EUR 2110.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Nicotinamide-N-Methyltransferase (NNMT) Protein

20-abx068293
  • EUR 662.00
  • EUR 272.00
  • EUR 2040.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Nicotinamide-N-Methyltransferase (NNMT) Protein

20-abx068294
  • EUR 662.00
  • EUR 272.00
  • EUR 2040.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide Riboside Kinase 1 (C9orf95) Antibody

abx034456-400ul 400 ul
EUR 523

Nicotinamide Riboside Kinase 1 (C9orf95) Antibody

abx034456-80l 80 µl
EUR 286

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody

20-abx003756
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Antibody

abx018035-100ug 100 ug
EUR 342

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1

7-03223 5µg Ask for price

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1

7-03224 20µg Ask for price

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1

7-03225 1mg Ask for price

Nicotinamide Nucleotide Adenylyltransferase 3 (Nmnat3) Antibody

abx432172-200ul 200 ul
EUR 384

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody

abx433042-200ul 200 ul
EUR 286

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody

abx433043-200ul 200 ul
EUR 384

Nicotinamide-N-Methyltransferase (NNMT) Antibody (FITC)

20-abx274230
  • EUR 495.00
  • EUR 258.00
  • EUR 1455.00
  • EUR 676.00
  • EUR 398.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody

20-abx317916
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody

20-abx320762
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody

20-abx320763
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

Nicotinamide N-Methyltransferase (NNMT) Antibody (HRP)

20-abx314889
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nicotinamide N-Methyltransferase (NNMT) Antibody (FITC)

20-abx314890
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Neutralizing mousemonoclonalantibodies (5F8 and 1A10) against human DKK2 have been efficiently developed by immunizing the mice with two completely different peptides (34KLNSIKSSL42 and 240KVWKDATYS248) conjugated to Keyhole limpet hemocyanin (KLH). The monoclonalantibodies not solely abolish DKK2’s suppression of Wnt signaling in vitro but additionally inhibits tumor development in vivo. Currently, these two mAbs are present process humanization as immunotherapy candidates and could provide a brand new drug for remedy of human cancers.

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