Rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal antibodies against DKK2 for cancer therapy.
Dickkopf-related protein 2 (DKK2)is a member of the Dickkopf household in Wnt signaling pathway. Recently, we discovered that antibodies against DKK2 might activate pure killer (NK) and CD8+ T cells in tumors and inhibit tumor development. In this paper, we report the rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal anti-DKK2 antibodies.
To break the immune tolerance, we designed and chemically synthesized six peptides akin to completely different areas of DKK2 as immunogens and discovered 5 of them might generate mouse polyclonal antibodies that may bind to the lively recombinant human DKK2 protein.
Description: A competitive ELISA for quantitative measurement of Canine Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.
Description: A competitive ELISA for quantitative measurement of Goat Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
ELISA kit for Human Nicotinamide N-methyltransferase
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Nicotinamide N-methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids.
General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Nicotinamide Adenine Dinucleotide (NAD) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Nicotinamide Adenine Dinucleotide (NAD) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Nicotinamide Adenine Dinucleotide (NAD) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Nicotinamide Adenine Dinucleotide (NAD) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Nicotinamide Adenine Dinucleotide (NAD) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human NNMT/ Nicotinamide N-methyltransferase ELISA Kit
Description: A competitive ELISA for quantitative measurement of Rabbit Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human NNMT(Nicotinamide N-methyltransferase) ELISA Kit
Description: A competitive ELISA for quantitative measurement of Monkey Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Nicotinamide N methyltransferase(NNMT) ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Nicotinamide N methyltransferase(NNMT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Nicotinamide N-methyltransferase(NNMT)ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in samples from serum, plasma or other biological fluids.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma, tissue homogenates and other biological fluids.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma, tissue homogenates and other biological fluids.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma, tissue homogenates and other biological fluids.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma, tissue homogenates and other biological fluids.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Nicotinamide-N-Methyltransferase (NNMT) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Nicotinamide-N-Methyltransferase (NNMT) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Nicotinamide-N-Methyltransferase (NNMT) ELISA Kit
Description: serum, plasma, tissue homogenates and other biological fluids.
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Neutralizing mousemonoclonalantibodies (5F8 and 1A10) against human DKK2 have been efficiently developed by immunizing the mice with two completely different peptides (34KLNSIKSSL42 and 240KVWKDATYS248) conjugated to Keyhole limpet hemocyanin (KLH). The monoclonalantibodies not solely abolish DKK2’s suppression of Wnt signaling in vitro but additionally inhibits tumor development in vivo. Currently, these two mAbs are present process humanization as immunotherapy candidates and could provide a brand new drug for remedy of human cancers.