Rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal antibodies against DKK2 for cancer therapy.

Dickkopf-related protein 2 (DKK2)is a member of the Dickkopf household in Wnt signaling pathway. Recently, we discovered that antibodies against DKK2 might activate pure killer (NK) and CD8+ T cells in tumors and inhibit tumor development. In this paper, we report the rational design of peptides for identification of linear epitopes and generation of neutralizing monoclonal anti-DKK2 antibodies.

To break the immune tolerance, we designed and chemically synthesized six peptides akin to completely different areas of DKK2 as immunogens and discovered 5 of them might generate mouse polyclonal antibodies that may bind to the lively recombinant human DKK2 protein.

Nicotinamide
GP9253-1KG	Glentham Life Sciences	1 kg	EUR 94

Nicotinamide
GP9253-250G	Glentham Life Sciences	250 g	EUR 54

Nicotinamide
GP9253-25G	Glentham Life Sciences	25 g	EUR 40

Nicotinamide
GP9253-500G	Glentham Life Sciences	500 g	EUR 70

Nicotinamide
N1651-5.1	ApexBio	10 mM (in 1mL DMSO)	EUR 142
Description: IC50: N/ANicotinamide is an inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. Poly(ADP-ribose) polymerase-1 (PARP-1) is found as a DNA repair enzyme.

Nicotinamide
N1651-50	ApexBio	50 mg	EUR 131
Description: IC50: N/ANicotinamide is an inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. Poly(ADP-ribose) polymerase-1 (PARP-1) is found as a DNA repair enzyme.

Nicotinamide
TB0209	ChemNorm	10X20mg	EUR 274

Nicotinamide [BSA]
DAG3371	Creative Diagnostics	1mg	EUR 845

?-Nicotinamide mononucleotide
2733-100	Biovision		EUR 349

?-Nicotinamide mononucleotide
2733-25	Biovision		EUR 164

?-Nicotinamide mononucleotide
2733-250	Biovision		EUR 631

Nicotinamide N-oxide
HY-101407	MedChemExpress	10mM/1mL	EUR 126

Nicotinamide riboside (chloride)
HY-123033A	MedChemExpress	100mg	EUR 108

β-Nicotinamide mononucleotide
HY-F0004	MedChemExpress	100mg	EUR 312

Beta-Nicotinamide Mononucleotide
abx188614-100g	Abbexa	100 g	EUR 1469

beta-Nicotinamide mononucleotide
GE9996-100MG	Glentham Life Sciences	100 mg	EUR 138

beta-Nicotinamide mononucleotide
B7878-100	ApexBio	100 mg	EUR 108

beta-Nicotinamide mononucleotide
B7878-500	ApexBio	500 mg	EUR 166

Beta-Nicotinamide Mononucleotide
TBW01655	ChemNorm	20mg	Ask for price

Nicotinamide (Vitamin B3) USP
N018-100G	TOKU-E	100 g	EUR 50

Nicotinamide (Vitamin B3) USP
N018-500G	TOKU-E	500 g	EUR 123

Nicotinamide N-Methyltransferase Antibody
20-abx114105	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Nicotinamide Nucleotide Transhydrogenase Antibody
20-abx114107	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Nicotinamide N-Methyltransferase (Recombinant)
20-abx073298	Abbexa	EUR 3418.00 EUR 328.00 EUR 230.00	1 mg 25 ug 5 ug

Nicotinamide Adenine Dinucleotide (BSA)
20-abx165783	Abbexa	EUR 634.00 EUR 272.00 EUR 1901.00 EUR 746.00 EUR 453.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide Adenine Dinucleotide (OVA)
20-abx165784	Abbexa	EUR 634.00 EUR 272.00 EUR 1901.00 EUR 746.00 EUR 453.00	100 ug 10 ug 1 mg 200 ug 50 ug

beta-Nicotinamide adenine dinucleotide
B7877-100	ApexBio	100 mg	EUR 108

beta-Nicotinamide adenine dinucleotide
B7877-500	ApexBio	500 mg	EUR 166

beta-Nicotinamide adenine dinucleotide
GV0860-10G	Glentham Life Sciences	10 g	EUR 174

beta-Nicotinamide adenine dinucleotide
GV0860-1G	Glentham Life Sciences	1 g	EUR 58

beta-Nicotinamide adenine dinucleotide
GV0860-25G	Glentham Life Sciences	25 g	EUR 329

beta-Nicotinamide adenine dinucleotide
GV0860-5G	Glentham Life Sciences	5 g	EUR 112

Nicotinamide Adenine Dinucleotide (NAD) Antibody
20-abx130456	Abbexa	EUR 425.00 EUR 133.00 EUR 1177.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx122668-100ug	Abbexa	100 ug	EUR 391

Nicotinamide Nucleotide Adenylyltransferase 1 Antibody
20-abx137248	Abbexa	EUR 704.00 EUR 328.00 EUR 230.00	100 ug 20 ug 5 ug

Nicotinamide-N-Methyltransferase (NNMT) Antibody
20-abx103358	Abbexa	EUR 425.00 EUR 133.00 EUR 1205.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide-N-Methyltransferase (NNMT) Antibody
20-abx103359	Abbexa	EUR 439.00 EUR 133.00 EUR 1233.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide-N-Methyltransferase (NNMT) Antibody
20-abx103360	Abbexa	EUR 439.00 EUR 133.00 EUR 1233.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide Nucleotide Adenylyltransferase 1 (Recombinant)
20-abx073291	Abbexa	EUR 3418.00 EUR 328.00 EUR 230.00	1 mg 20 ug 5 ug

Nicotinamide Nucleotide Adenylyltransferase 2 (Recombinant)
20-abx073965	Abbexa	EUR 1609.00 EUR 328.00 EUR 230.00	100 ug 10 ug 2 µg

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody
20-abx007786	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody
20-abx003424	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Nicotinamide N-Methyltransferase (NNMT) Antibody
20-abx005116	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx025652-400ul	Abbexa	400 ul	EUR 523

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx025652-80l	Abbexa	80 µl	EUR 286

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx027598-400ul	Abbexa	400 ul	EUR 523

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx027598-80l	Abbexa	80 µl	EUR 286

Recombinant Human Nicotinamide N-Methyltransferase
7-03229	CHI Scientific	5µg	Ask for price

Recombinant Human Nicotinamide N-Methyltransferase
7-03230	CHI Scientific	25µg	Ask for price

Recombinant Human Nicotinamide N-Methyltransferase
7-03231	CHI Scientific	1mg	Ask for price

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx431457-200ul	Abbexa	200 ul	EUR 384

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx433044-200ul	Abbexa	200 ul	EUR 384

Nicotinamide N-Methyltransferase (NNMT) Antibody
20-abx301014	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Nicotinamide N-Methyltransferase (NNMT) Antibody
20-abx321820	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

N-Benzyl-5-bromo-nicotinamide
20-abx186522	Abbexa	EUR 217.00 EUR 648.00 EUR 328.00	1 g 25 g 5 g

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody
20-abx217198	Abbexa	EUR 425.00 EUR 342.00	100 ug 50 ug

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody
abx146315-100ug	Abbexa	100 ug	EUR 391

Nicotinamide N-Methyltransferase (NNMT) Antibody
abx235773-100ug	Abbexa	100 ug	EUR 509

Nicotinamide Nucleotide Transhydrogenase (NNT) Antibody
abx235775-100ug	Abbexa	100 ug	EUR 481

Nicotinamide N-Methyltransferase (NNMT) Antibody
20-abx141366	Abbexa	EUR 370.00 EUR 606.00 EUR 300.00	100 ul 200 ul 50 ul

Nicotinamide-N-Methyltransferase (NNMT) Antibody
20-abx173825	Abbexa	EUR 857.00 EUR 439.00	1 mg 200 ug

Human Nicotinamide N-methyltransferase (NNMT)
1-CSB-EP015906HU	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Nicotinamide N-methyltransferase(NNMT) expressed in E.coli

Recombinant Nicotinamide-N-Methyltransferase (NNMT)
4-RPH530Hu01	Cloud-Clone	EUR 490.66 EUR 234.00 EUR 1564.96 EUR 588.32 EUR 1076.64 EUR 391.00 EUR 3762.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Nicotinamide-N-Methyltransferase expressed in: E.coli

Recombinant Nicotinamide-N-Methyltransferase (NNMT)
4-RPH530Mu01	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Nicotinamide-N-Methyltransferase expressed in: E.coli

Recombinant Nicotinamide-N-Methyltransferase (NNMT)
4-RPH530Mu02	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Nicotinamide-N-Methyltransferase expressed in: E.coli

?-Nicotinamide adenine dinucleotide sodium salt
9457-1G	Biovision		EUR 109

?-Nicotinamide adenine dinucleotide sodium salt
9457-25G	Biovision		EUR 762

?-Nicotinamide adenine dinucleotide sodium salt
9457-5G	Biovision		EUR 251

Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit
20-abx585168	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

NNMTMouse Nicotinamide N-Methyltransferase MouseRecombinant Protein
PROTO55239	BosterBio	Regular: 10ug	EUR 317
Description: NNMT Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids (1-264a.a) and having a molecular mass of 32.1kDa.;NNMT is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Pig NAMPT/ Nicotinamide phosphoribosyltransferase ELISA Kit
E0138Pi	Sunlong	1 Kit	EUR 717

Mouse Nampt/ Nicotinamide phosphoribosyltransferase ELISA Kit
E1008Mo	Sunlong	1 Kit	EUR 571

Rat Nampt/ Nicotinamide phosphoribosyltransferase ELISA Kit
E0659Ra	Sunlong	1 Kit	EUR 571

Human NAMPT/ Nicotinamide phosphoribosyltransferase ELISA Kit
E2812Hu	Sunlong	1 Kit	EUR 563

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody
abx122119-100ug	Abbexa	100 ug	EUR 391

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody
abx122669-100ug	Abbexa	100 ug	EUR 391

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody
20-abx114106	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Human Nicotinamide-N-Methyltransferase (NNMT) Protein
20-abx068292	Abbexa	EUR 690.00 EUR 286.00 EUR 2110.00 EUR 815.00 EUR 495.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Nicotinamide-N-Methyltransferase (NNMT) Protein
20-abx068293	Abbexa	EUR 662.00 EUR 272.00 EUR 2040.00 EUR 787.00 EUR 481.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Nicotinamide-N-Methyltransferase (NNMT) Protein
20-abx068294	Abbexa	EUR 662.00 EUR 272.00 EUR 2040.00 EUR 787.00 EUR 481.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide Riboside Kinase 1 (C9orf95) Antibody
abx034456-400ul	Abbexa	400 ul	EUR 523

Nicotinamide Riboside Kinase 1 (C9orf95) Antibody
abx034456-80l	Abbexa	80 µl	EUR 286

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody
20-abx003756	Abbexa	EUR 411.00 EUR 592.00	100 ul 200 ul

Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Antibody
abx018035-100ug	Abbexa	100 ug	EUR 342

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1
7-03223	CHI Scientific	5µg	Ask for price

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1
7-03224	CHI Scientific	20µg	Ask for price

Recombinant Human Nicotinamide Nucleotide Adenylyltransferase 1
7-03225	CHI Scientific	1mg	Ask for price

Nicotinamide Nucleotide Adenylyltransferase 3 (Nmnat3) Antibody
abx432172-200ul	Abbexa	200 ul	EUR 384

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody
abx433042-200ul	Abbexa	200 ul	EUR 286

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody
abx433043-200ul	Abbexa	200 ul	EUR 384

Nicotinamide-N-Methyltransferase (NNMT) Antibody (FITC)
20-abx274230	Abbexa	EUR 495.00 EUR 258.00 EUR 1455.00 EUR 676.00 EUR 398.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) Antibody
20-abx317916	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody
20-abx320762	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) Antibody
20-abx320763	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Nicotinamide N-Methyltransferase (NNMT) Antibody (HRP)
20-abx314889	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Nicotinamide N-Methyltransferase (NNMT) Antibody (FITC)
20-abx314890	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Neutralizing mousemonoclonalantibodies (5F8 and 1A10) against human DKK2 have been efficiently developed by immunizing the mice with two completely different peptides (³⁴KLNSIKSSL⁴² and ²⁴⁰KVWKDATYS²⁴⁸) conjugated to Keyhole limpet hemocyanin (KLH). The monoclonalantibodies not solely abolish DKK2’s suppression of Wnt signaling in vitro but additionally inhibits tumor development in vivo. Currently, these two mAbs are present process humanization as immunotherapy candidates and could provide a brand new drug for remedy of human cancers.