Multidrug-resistant enterococci are main causes of hospital-acquired infections. Immunotherapy with monoclonal antibodies (MAbs) concentrating on bacterial antigens could be a worthwhile remedy choice on this setting.
Here, we describe the event of two MAbs by way of hybridoma expertise that concentrate on antigens from essentially the most clinically related enterococcal species.
Diheteroglycan (DHG), a well-characterized capsular polysaccharide of Enterococcus faecalis, and the secreted antigen A (SagA), an immunogenic protein from Enterococcus faecium, are each immunogens which were confirmed to boost opsonic and cross-reactive antibodies against enterococcal strains.
Development of Opsonic Mouse Monoclonal Antibodies against Multidrug-Resistant Enterococci.
For this objective, a conjugated type of the native DHG with SagA was used to boost the antibodies in mice, whereas enzyme-linked immunosorbent assay and opsonophagocytic assay had been mixed within the choice course of of hybridoma cells producing immunoreactive and opsonic antibodies concentrating on the chosen antigens.
Comparisons between Mnoclonal, Polyclonal and Recombinant antibodies and their benefits: Regular monoclonal antibodies have higher purity, better specificity and less lot-to-lot variations than polyclonal antibodies. Recombinant antibodies, however,
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transc
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transc
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transc
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transc
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Nuclear factor-KB p65 (NF-KB p65)
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transc
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Nuclear factor-KB p65 (NF-KB p65)
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transc
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NF-KB p100 (Ab-869). This antibody is tested and proven to work in the following applications:
Description: A Mouse Monoclonal antibody against Acetyl NF kB P65?K314/K315) (5G11) from Human/ Mouse/ Rat. This antibody is tested and validated for IHC
Description: A Mouse Monoclonal antibody against Acetyl NF kB P65?K314/K315) (5G11) from Human/ Mouse/ Rat. This antibody is tested and validated for IHC
Immunogen information: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)
Applications tips:
Description: A monoclonal antibody for detection of Acetyl NF kB P65 K314/K315) from Human, Mouse, Rat. This Acetyl NF kB P65 K314/K315) antibody is for IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide of Acetyl NF kB P65 (K314/K315)
Immunogen information: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)
Applications tips:
Description: A monoclonal antibody for detection of Acetyl NF kB P65 K314/K315) from Human, Mouse, Rat. This Acetyl NF kB P65 K314/K315) antibody is for IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide of Acetyl NF kB P65 (K314/K315)
Immunogen information: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)
Applications tips:
Description: A monoclonal antibody for detection of Acetyl NF kB P65 K314/K315) from Human, Mouse, Rat. This Acetyl NF kB P65 K314/K315) antibody is for IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide of Acetyl NF kB P65 (K314/K315)
Immunogen information: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)
Applications tips:
Description: A monoclonal antibody for detection of Acetyl NF kB P65 K314/K315) from Human, Mouse, Rat. This Acetyl NF kB P65 K314/K315) antibody is for IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide of Acetyl NF kB P65 (K314/K315)
Immunogen information: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)
Applications tips:
Description: A monoclonal antibody for detection of Acetyl NF kB P65 K314/K315) from Human, Mouse, Rat. This Acetyl NF kB P65 K314/K315) antibody is for IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide of Acetyl NF kB P65 (K314/K315)
Immunogen information: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)
Applications tips:
Description: A monoclonal antibody for detection of Acetyl NF kB P65 K314/K315) from Human, Mouse, Rat. This Acetyl NF kB P65 K314/K315) antibody is for IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide of Acetyl NF kB P65 (K314/K315)
ELISA kit for Human Nuclear factor-kappa B (NF-kB)
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-kappa B (NF-kB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Nuclear factor-kappa B (NF-kB)
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-kappa B (NF-kB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Nuclear factor-kappa B (NF-kB)
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-kappa B (NF-kB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a D
Description: Quantitative sandwich ELISA for measuring Rat Nuclear factor-kappa B (NF-kB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a D
Description: Quantitative sandwich ELISA for measuring Rat Nuclear factor-kappa B (NF-kB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a D
Description: Quantitative sandwich ELISA for measuring Rat Nuclear factor-kappa B (NF-kB) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat pI?B? (Phospho-Inhibitory Subunit of NF-KB?)
Gentaur's pI?B? ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat pI?B?. Standards or samples are added to the micro ELISA plate wells and combined with th
Description: A sandwich ELISA kit for quantitative measurement of Rat pI?B? (Phospho-Inhibitory Subunit of NF-KB?) in samples from Serum, Plasma, Cell supernatant
NF-kB p50 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
From this course of, two extremely particular IgG1(κ) MAbs had been obtained, one against the polysaccharide (DHG.01) and one against the protein (SagA.01). Both MAbs exhibited good opsonic killing against the goal bacterial strains: DHG.01 confirmed 90% killing against E. faecalis kind 2, and SagA.01 confirmed 40% killing against E. faecium 11231/6.
In addition, each MAbs confirmed cross-reactivity towards different E. faecalis and E. faecium strains. The sequences from the variable areas of the heavy and light-weight chains had been reconstructed in expression vectors, and the exercise of the MAbs upon expression in eukaryotic cells was confirmed with the identical immunological assays. In abstract, we recognized two opsonic MAbs against enterococci which may very well be used for therapeutic or prophylactic approaches against enterococcal infections.
)
)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
 Antibody)
+ EF1-Neo)
+ EF1-Puro)
 + EF1-Neo)
 + EF1-Puro)
 Antibody)
 Antibody)
 Antibody)
 antibody)
 CLIA Kit)
)
)
 Conjugated Antibody)
 Blocking Peptide)
 Blocking Peptide)
 Blocking Peptide)
)
)
 antibody)
 CLIA Kit)
 (5G11) Monoclonal Antibody)
 (Target 1))
 (Target 2))
 (Target 3))
 CLIA Kit)
 Mouse Monoclonal Antibody (2A11))
 Mouse Monoclonal Antibody (5G11))
)
 Mouse Monoclonal Antibody(2A11))
 Mouse Monoclonal Antibody(5G11))
)
)
)
)
 (Target 1))
 (Target 2))
 (Target 3))
