Development of Opsonic Mouse Monoclonal Antibodies against Multidrug-Resistant Enterococci.

Multidrug-resistant enterococci are main causes of hospital-acquired infections. Immunotherapy with monoclonal antibodies (MAbs) concentrating on bacterial antigens could be a worthwhile remedy choice on this setting.

Here, we describe the event of two MAbs by way of hybridoma expertise that concentrate on antigens from essentially the most clinically related enterococcal species.

Diheteroglycan (DHG), a well-characterized capsular polysaccharide of Enterococcus faecalis, and the secreted antigen A (SagA), an immunogenic protein from Enterococcus faecium, are each immunogens which were confirmed to boost opsonic and cross-reactive antibodies against enterococcal strains.

Development of Opsonic Mouse Monoclonal Antibodies against Multidrug-Resistant Enterococci.
Development of Opsonic Mouse Monoclonal Antibodies against Multidrug-Resistant Enterococci.

For this objective, a conjugated type of the native DHG with SagA was used to boost the antibodies in mice, whereas enzyme-linked immunosorbent assay and opsonophagocytic assay had been mixed within the choice course of of hybridoma cells producing immunoreactive and opsonic antibodies concentrating on the chosen antigens.

NF-kB p65 Antibody

DF7003 200ul
EUR 304
Description: p65 Antibody detects endogenous levels of total p65.

pGreenFire1-NF-kB (plasmid)

TR012PA-1 10 ug
EUR 786

pGreenFire1-NF-kB (virus)

TR012VA-1 >2 x 10^6 IFUs
EUR 786

NF-kB p65 Mouse mAb

A10609-100ul 100 ul
EUR 384

NF-kB p65 Mouse mAb

A10609-200ul 200 ul
EUR 554

NF-kB p65 Mouse mAb

A10609-20ul 20 ul Ask for price

NF-kB p65 Mouse mAb

A10609-50ul 50 ul
EUR 265

NF-kB p65 Rabbit pAb

A11202-100ul 100 ul
EUR 308

NF-kB p65 Rabbit pAb

A11202-200ul 200 ul
EUR 459

NF-kB p65 Rabbit pAb

A11202-20ul 20 ul
EUR 183

NF-kB p65 Rabbit pAb

A11202-50ul 50 ul
EUR 223

NF-kB p65 Rabbit pAb

A11204-100ul 100 ul
EUR 308

NF-kB p65 Rabbit pAb

A11204-200ul 200 ul
EUR 459

NF-kB p65 Rabbit pAb

A11204-20ul 20 ul
EUR 183

NF-kB p65 Rabbit pAb

A11204-50ul 50 ul
EUR 223

NF-kB p100 (pS872) Antibody

abx011250-100ug 100 ug
EUR 439

NF-kB p65 (pS281) Antibody

abx011264-100ug 100 ug
EUR 439

NF-kB p65 (pS529) Antibody

abx011265-100ug 100 ug
EUR 439

NF-kB p65 (pS311) Antibody

abx011266-100ug 100 ug
EUR 439

NF-kB p65 (pS536) Antibody

abx011269-100ug 100 ug
EUR 439

NF-kB p65 (pS276) Antibody

abx011270-100ug 100 ug
EUR 439

NF-kB p65 (pS468) Antibody

abx011271-100ug 100 ug
EUR 439

NF-kB p65 (pT254) Antibody

abx011272-100ug 100 ug
EUR 439

NF-kB p65 (pT435) Antibody

abx011273-100ug 100 ug
EUR 439

NF-kB p65 Rabbit pAb

A0450-100ul 100 ul
EUR 308

NF-kB p65 Rabbit pAb

A0450-200ul 200 ul
EUR 459

NF-kB p65 Rabbit pAb

A0450-20ul 20 ul Ask for price

NF-kB p65 Rabbit pAb

A0450-50ul 50 ul Ask for price

NF-kB p65 Rabbit pAb

A16271-100ul 100 ul
EUR 308

NF-kB p65 Rabbit pAb

A16271-200ul 200 ul
EUR 459

NF-kB p65 Rabbit pAb

A16271-20ul 20 ul
EUR 183

NF-kB p65 Rabbit pAb

A16271-50ul 50 ul
EUR 223

NF-kB p65 Rabbit pAb

A2547-100ul 100 ul
EUR 308

NF-kB p65 Rabbit pAb

A2547-200ul 200 ul
EUR 459

NF-kB p65 Rabbit pAb

A2547-20ul 20 ul
EUR 183

NF-kB p65 Rabbit pAb

A2547-50ul 50 ul
EUR 223

NF-kB p65 Conjugated Antibody

C48676 100ul
EUR 397

NF-kB p65 Mouse mAb

A10609 20 μL
EUR 15

NF-kB p65 Blocking Peptide

BF0382-BP 1mg
EUR 195

NF-kB p65 Blocking Peptide

AF0874-BP 1mg
EUR 195

NF-kB p65 Blocking Peptide

DF7003-BP 1mg
EUR 195

Anti-NF-kB p65/RELA Antibody

PA1125 100ug/vial
EUR 294

Anti-NF-kB p65/RELA Antibody

PA1669 100ug/vial
EUR 334

Anti-NF-kB p65/RELA Antibody

PB9087 100ug/vial
EUR 294

Anti-NF-kB p65/RELA Antibody

PB9324 100ug/vial
EUR 294

Anti-NF-kB p65/RELA Antibody

A00284-1 100ug/vial
EUR 334

NF-kB p100 / p52 (pS865) Antibody

abx011251-100ug 100 ug
EUR 439

NF-kB p100 / p52 (pS869) Antibody

abx011252-100ug 100 ug
EUR 439

NF-kB p105 / p50 (pS932) Antibody

abx011254-100ug 100 ug
EUR 439

NF-kB p105 / p50 (pS893) Antibody

abx011255-100ug 100 ug
EUR 439

NF-kB p105 / p50 (pS337) Antibody

abx011256-100ug 100 ug
EUR 439

NF-kB p105 / p50 (pS927) Antibody

abx011257-100ug 100 ug
EUR 439

NF-kB p105 / p50 (pS907) Antibody

abx011259-100ug 100 ug
EUR 439

Anti-NF-kB p65 (Ser316) antibody

STJ120299 100 µl
EUR 526

NF-kB p65 (Phospho-S529) Antibody

13347-100ul 100ul
EUR 333

NF-kB p65 (Phospho-S529) Antibody

13347-50ul 50ul
EUR 239

Nuclear Factor NF-KB Inhibitor SN50

H-6052.0500 0.5mg
EUR 273
Description: Sum Formula: C129H230N36O29S; CAS# [213546-53-3] net

Nuclear Factor NF-KB Inhibitor SN50

H-6052.1000 1.0mg
EUR 454
Description: Sum Formula: C129H230N36O29S; CAS# [213546-53-3] net

NF-kB p65 Polyclonal Conjugated Antibody

C27518 100ul
EUR 397

Phospho-NF-kB p105 (Ser903) Antibody

AF8013 200ul
EUR 376
Description: NF-κB p105 (Phospho-Ser903) Antibody detects endogenous levels of NF-κB p105 only when phosphorylated at Ser903.

Phospho-NF-kB p105 (Ser923) Antibody

AF8014 200ul
EUR 376
Description: NF-κB p105 (Phospho-Ser923) Antibody detects endogenous levels of NF-κB p105 only when phosphorylated at Ser923.

Phospho-NF-kB p100 (Ser872) Antibody

AF3375 200ul
EUR 304
Description: Phospho-NF-κB p100 (Ser872) Antibody detects endogenous levels of NF-κB p100 only when phosphorylated at Serine 872.

NF-kB p65 recombinant monoclonal antibody

A5075 100ul X 3
EUR 595
Description: A recombinant monoclonal antibody from rabbit against human NF-kB p65 for WB, IHC, IF,ELISA

pGreenFire1-NF-kB (plasmid)+ EF1-Neo

TR012PA-N 10 ug
EUR 786

pGreenFire1-NF-kB (plasmid)+ EF1-Puro

TR012PA-P 10 ug
EUR 786

pGreenFire1-NF-kB (virus) + EF1-Neo

TR012VA-N >2 x 10^6 IFUs
EUR 786

pGreenFire1-NF-kB (virus) + EF1-Puro

TR012VA-P >2 x 10^6 IFUs
EUR 786

pGL4.32[luc2P/NF-kB-RE/Hygro]

PVT13580 2 ug
EUR 703

Rat Nuclear Factor-kB P65 (NF-kB p65) CLIA Kit

abx196083-96tests 96 tests
EUR 825

ELISA kit for Mouse Nuclear factor-KB p65 (NF-KB p65)

KTE70510-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Mouse Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Nuclear factor-KB p65 (NF-KB p65)

KTE70510-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Mouse Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Nuclear factor-KB p65 (NF-KB p65)

KTE70510-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Mouse Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Nuclear factor-KB p65 (NF-KB p65)

KTE60811-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Nuclear factor-KB p65 (NF-KB p65)

KTE60811-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Nuclear factor-KB p65 (NF-KB p65)

KTE60811-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Human Nuclear factor-KB p65 (NF-KB p65) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Phospho-NF-kB p65-T254 Rabbit pAb

AP0122-100ul 100 ul
EUR 384

Phospho-NF-kB p65-T254 Rabbit pAb

AP0122-200ul 200 ul
EUR 554

Phospho-NF-kB p65-T254 Rabbit pAb

AP0122-20ul 20 ul Ask for price

Phospho-NF-kB p65-T254 Rabbit pAb

AP0122-50ul 50 ul
EUR 265

Phospho-NF-kB p65-S276 Rabbit pAb

AP0123-100ul 100 ul
EUR 384

Phospho-NF-kB p65-S276 Rabbit pAb

AP0123-200ul 200 ul
EUR 554

Phospho-NF-kB p65-S276 Rabbit pAb

AP0123-20ul 20 ul
EUR 183

Phospho-NF-kB p65-S276 Rabbit pAb

AP0123-50ul 50 ul
EUR 265

Phospho-NF-kB p65-S536 Rabbit pAb

AP0124-100ul 100 ul
EUR 384

Phospho-NF-kB p65-S536 Rabbit pAb

AP0124-200ul 200 ul
EUR 554

Phospho-NF-kB p65-S536 Rabbit pAb

AP0124-20ul 20 ul
EUR 183

Phospho-NF-kB p65-S536 Rabbit pAb

AP0124-50ul 50 ul
EUR 265

Phospho-NF-kB p65-T505 Rabbit pAb

AP0190-100ul 100 ul
EUR 384

Phospho-NF-kB p65-T505 Rabbit pAb

AP0190-200ul 200 ul
EUR 554

Phospho-NF-kB p65-T505 Rabbit pAb

AP0190-20ul 20 ul Ask for price

Phospho-NF-kB p65-T505 Rabbit pAb

AP0190-50ul 50 ul
EUR 265

Phospho-NF-kB p65-S529 Rabbit pAb

AP0215-100ul 100 ul
EUR 384

Phospho-NF-kB p65-S529 Rabbit pAb

AP0215-200ul 200 ul
EUR 554

Phospho-NF-kB p65-S529 Rabbit pAb

AP0215-20ul 20 ul
EUR 183

Phospho-NF-kB p65-S529 Rabbit pAb

AP0215-50ul 50 ul
EUR 265

Phospho-NF-kB p65-S311 Rabbit pAb

AP0445-100ul 100 ul
EUR 384

From this course of, two extremely particular IgG1(κ) MAbs had been obtained, one against the polysaccharide (DHG.01) and one against the protein (SagA.01). Both MAbs exhibited good opsonic killing against the goal bacterial strains: DHG.01 confirmed 90% killing against E. faecalis kind 2, and SagA.01 confirmed 40% killing against E. faecium 11231/6.

In addition, each MAbs confirmed cross-reactivity towards different E. faecalis and E. faecium strains. The sequences from the variable areas of the heavy and light-weight chains had been reconstructed in expression vectors, and the exercise of the MAbs upon expression in eukaryotic cells was confirmed with the identical immunological assays. In abstract, we recognized two opsonic MAbs against enterococci which may very well be used for therapeutic or prophylactic approaches against enterococcal infections.

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